OngletsPlateforme Description High troughput screening (Luminescence, Fluorescence and absorbance assays) Assay development and miniaturisation for HTS Chemical siRNA transfection protocols for >20 different ATCC adherent cell lines, immortalized primary cell lines & primary cells, mouse ESC, L2 biosafety level micro-organisms Virus mediated gene transfer (lentivirus, adenovirus) High Content Analysis of multiple biochemical and morphological parameters in cellular systems (integration up to 50 parameters) Statistical data analysis HTS project management; HTS-related projects funding application Descriptif des prestations Screening of compounds (siRNA, cDNA, molecules) Target genes identification Hit identification and validation Moyens et équipements High Throughput Screening systems with robotic micro-plate manipulation and automated acquisition (TECAN EVO150-PW384; INCell 1000; Mithras LB940) High Throughput Screening system in L2 Biosafety environment siRNA, cDNA and Chemical compounds libraries Site internet de la plateforme http://www.igbmc.fr/technologies/5/team/56/ Langue Français